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| 产品名称： | c8082 |
|---|---|
| 商品货号： | TS180234 |
| Designations： | c8082 |
| GenBank Number： | U00062 U10399 U10400 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | L Riles, MV Olson |
| Insert： | DNA: genomic Insert lengths(kb): 37.64199829101563 Gene product: myosin ARG4 |
| Insert Size (kb)： | 37.642 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--6.6, 5.0, 3.1, 3.0, 2.7 (doublet), 2.3, 1.9, 1.8 (doublet), 1.3, 1.2, 1.0 (doublet), 0.9 (doublet), 0.85, 0.8 (doublet), 0.75, 0.55 (doublet), 0.4. Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 6.732, 1.006 (vector); 5.10, 3.165, 2.942, 2.79, 2.648, 2.34, 1.892, 1.825, 1.766, 1.277, 1.141, 1.059 (2), 0.917 (2), 0.873, 0.814, 0.771, 0.767, 0.712, 0.526, 0.485, 0.381. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 139499-177141 of the sequence of chromosome VIII. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229 Linda Riles, personal communication |