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| 产品名称： | pBS39 BSY90 |
|---|---|
| 商品货号： | TS180285 |
| Designations： | pBS39 BSY90 |
| GenBank Number： | X03453 |
| Species： | Staphylococcus aureus subsp.aureus bacteriophage P1 |
| Depositors： | DuPont Merck Pharmaceutical Company, BL Sauer, DuPont Merck Pharmaceutical Company |
| Applications： | in another host, produces protein recombinase, site-specific |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: unknown Intact vector size: Type of vector: plasmid Vector end: SalI Vector end: XhoI Cloning sites: Polylinker sites: Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): ampR, URA3 Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: bacteriophage P1 Gene symbol: cre Gene name: recombinase, site-specific Contains complete coding sequence?: U Type of DNA: genomic Insert 5 end: XhoI Insert 3 end: SalI Insert size (kb): 1.2 Cross references: DNA Seq. Acc.: X03453 Insert lengths(kb): 1.200000047683716 Gene product: recombinase, site-specific cre Target Gene: recombinase, site-specific |
| Insert Size (kb)： | 1.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI/XhoI--4.1 (doublet), 1.5; SalI--9.6; XhoI--5.5, 4.1. Permits inducible expression of cre in S. cerevisiae regulated by the GAL1 promoter. The order of the major features in this plasmid is: ampR - GAL1 promoter - cre - URA3 - ARS1 - CEN4. |
| References： | Sauer BL. Site-specific recombination of DNA in eukaryotic cells. US Patent 4,959,317 dated Sep 25 1990 Sauer B. Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 7: 2087-2096, 1987. PubMed: 3037344 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |