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微信小章| 产品名称： | pIP62 |
|---|---|
| 商品货号： | TS180300 |
| Designations： | pIP62 |
| GenBank Number： | L12374 |
| Species： | Drosophila melanogaster, fruit fly |
| Depositors： | WJ Van De Ven |
| Applications： | in another host, produces protein Dfurin 1 |
| Vector： | Construct size (kb): 7.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.5 Tissue: embryo Gene product: Dfurin 1 Dfur1 Target Gene: Dfurin 1 |
| Insert Size (kb)： | 4.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.5, 2.35, 0.85, 0.4; NotI--4.8, 2.35; BamHI--3.8, 2.5, 0.9; SalI--4.3, 2.9; PvuI--1.95 (doublet), 1.6, 1.2, 0.5. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--1.26, 3.70; EcoRI--3.32, 3.68, 3.75; SalI--2.74. Encodes the X isoform (7.6 kb transcript). Contains the complete coding sequence (nt 713-4522). Corresponds exactly to GenBank accession L12374. |
| References： | Van de Ven WJ, et al. Furin: the prototype mammalian subtilisin-like proprotein-processing enzyme. Endoproteolytic cleavage at paired basic residues of proproteins of the eukaryotic secretory pathway. Enzyme 45: 257-270, 1991. PubMed: 1843280 Roebroek AJ, et al. Generation of structural and functional diversity in furin-like proteins in Drosophila melanogaster by alternative splicing of the Dfur1 gene. EMBO J. 12: 1853-1870, 1993. PubMed: 8491178 Wim J Van De Ven, personal communication |