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| 产品名称： | alpha4 UTR-00723, WI-9025 |
|---|---|
| 商品货号： | TS180397 |
| Designations： | alpha4 UTR-00723, WI-9025 |
| GenBank Number： | X16983 |
| Species： | Homo sapiens, human |
| Depositors： | Q Yuan, ME Hemler |
| Vector： | Construct size (kb): 6.769999980926514 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript KS- Intact vector size: 2.964 Type of vector: phagemid Vector end: EcoRI closer to SalI Vector end: EcoRI Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI Polylinker sites: SEE COMMENTS Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ITGA4 Gene name: integrin, alpha 4 (CD49D, alpha 4 subunit of VLA-4 receptor) Contains complete coding sequence?: Y Chromosome: 2; Localization: 2 q31-q32; 2, 913.240000 cR Tissue: HPB-mLT cell line Disease: leukemia, T-cell Type of DNA: cDNA Insert end: Modification: EcoRI linker Insert end: Modification: EcoRI linker Insert size (kb): 3.805 Cross references: DNA Seq. Acc.: X16983 Nucleotides 1-3805 of the insert correspond to nucleotides 1-3805 of X16983. Insert lengths(kb): 3.805000066757202 Tissue: HPB-MLT cell line leukemia, T-cell Disease: leukemia, T-cell Gene product: integrin, alpha 4 (CD49D, alpha 4 subunit of VLA-4 receptor)(integrin, alpha 4) ITGA4 |
| Insert Size (kb)： | 3.805 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | Purified plasmid DNA of this item is available as ATCC#95495. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI/XbaI--3.8, 2.9; BamHI--3.3, 3.3; HindIII--5.6, 1.0; EcoRI--3.0, 2.0, 1.8; XbaI--6.7. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--1.83; HindIII--0.94 EcoRI--1.83. |
| References： | Takada Y, et al. The primary structure of the alpha 4 subunit of VLA-4: homology to other integrins and a possible cell-cell adhesion function. EMBO J. 8: 1361-1368, 1989. PubMed: 2788572 |