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| 产品名称： | C122/U3-T1 C C122/T1, C122/U3-T1 |
|---|---|
| 商品货号： | TS180474 |
| Designations： | C122/U3-T1 C C122/T1, C122/U3-T1 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff |
| Vector： | Construct size (kb): 27.60000038146973 |
| Insert： | DNA: genomic Insert lengths(kb): 20.0 Gene product: methionyl-tRNA synthetase MSM1 |
| Insert Size (kb)： | 20.0 |
| Genotype： | MATalpha ura3-1 msm1-1 |
| Mating Type： | alpha |
| Media： | ATCC® Medium 1688: Glycerol enriched medium |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following sites separated by (kb) (approximately): SacI- 0.3- KpnI- 1.3- KpnI- 1.0- BamHI- 1.2- XbaI- 0.8- BamHI- 0.4- PstI- 0.4- XbaI- 0.4- BamHI- 0.4- PstI- 0.4- KpnI- 0.5- SacI. Carries plasmid pG72/T1 with wild-type gene MSM1 is the nuclear gene encoding the mitochondrial methionyl-tRNA synthetase. The smallest region capable of complementing a msm1-1 mutation is defined by a KpnI-PstI fragment of approximately 3 kb from the middle of the insert, and spanning the BamHI and XbaI sites separated by 1.2 kb. The probe, pG72/T1, has been deposited in Escherichia coli RR1 as ATCC 37663 and in Saccharomyces cerevisiae C122/U3 as TS180474. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Tzagoloff A, et al. Characterization of MSM1, the structural gene for yeast mitochondrial methionyl-tRNA synthetase. Eur. J. Biochem. 179: 365-371, 1989. PubMed: 2645139 |