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| 产品名称： | pKB112 |
|---|---|
| 商品货号： | TS180542 |
| Designations： | pKB112 |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.1999998092651400 Vector: pKB112 (plasmid) Promoters: Promoter CYC1 promoter (including CYC1 UAS) Construction: pBR322 Marker(s):URA3,ampR Construct size (kb): 10.19999980926514 Features: marker(s): URA3 marker(s): ampR promoter: CYC1 promoter (including CYC1 UAS) replicon: 2 micron ori replicon: pMB1 coding sequence: lacZ |
| Applications： | YE-type (episomal) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): KpnI--10.3; HindIII--8.2, 2.1; SalI--10.3. This vector is one of three lacZ reporter plasmids that contain UAS (upstream activation site) elements (ATCC 87511, 87512 & 87522). They differ by polylinker sites. DNA fragments can be inserted into these plasmids to screen for DNA elements that reduce promoter activity. The vector was constructed by Aaron Mitchell. |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Bowdish KS, Mitchell AP. Bipartite structure of an early meiotic upstream activation sequence from Saccharomyces cerevisiae. Mol. Cell. Biol. 13: 2172-2181, 1993. PubMed: 8455605 |
| Related Products： | component of:ATCC 87540 |
| Shipped： | freeze-dried |