微信小陈
微信小章
产品简介
购买须知
| 产品名称： | c08H4 |
|---|---|
| 商品货号： | TS180574 |
| Designations： | c08H4 |
| Species： | Homo sapiens, human |
| Depositors： | PI Patel |
| Applications： | The 7.5 kb EcoRI fragment of the insert has been used to detect the TaqI RFLP at D17S122 and the dosage difference indicative of the duplication mutation associated with Charcot-Marie-Tooth disease (CMT1A). |
| Vector： | Construct size (kb): 36.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: SuperCos 1 Intact vector size: 7.600 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI Polylinker sites: EcoRI NotI BamHI NotI EcoRI Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR, neoR, G418R promoter: T3, T7 replicon: pMB1, SV40 Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D17S122 Genomic copy number: unique Gene name: DNA Segment, single copy Contains complete coding sequence?: U Chromosome: 17; Localization: 17 p12-p11.2 Type of DNA: genomic Insert end: Insert end: Insert size (kb): 29.0 Cross references: Insert lengths(kb): 29.0 Gene product: DNA Segment, single copy D17S122 Alleles: A1, A1, A2, A3, B1, A2, B2 |
| Insert Size (kb)： | 29.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--9.4, 7.6, 7.5, 4.4, 2.6, 1.55, 1.45 (doublet); NotI--9.4 (doublet), 7.6, 1.45; BamHI--11.5, 8.0, 6.6, 5.2, 3.5; EcoRI/BamHI--7.6, 7.5, 5.4, 3.0, 2.7, 2.15, 1.6, 1.45 (doublet); SacII--uncut. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The 7.5 kb EcoRI fragment of the insert has been used to detect the TaqI RFLP at D17S122 and the dosage difference indicative of the duplication mutation associated with Charcot-Marie-Tooth disease (CMT1A). |
| References： | Lupski JR, et al. DNA duplication associated with Charcot-Marie-Tooth disease type 1A. Cell 66: 219-232, 1991. PubMed: 1677316 Pragna I Patel, personal communication |