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微信小章| 产品名称： | pSM143 |
|---|---|
| 商品货号： | TS180583 |
| Designations： | pSM143 |
| Depositors： | G Grandi |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 |
| Vector Information： | Size (kb): 7.3499999046325680 Vector: pSM143 (plasmid) Promoters: Promoter ErmC Construction: pUB110, pBR322 ori, fd terminator Marker(s):ampR,kanR Construct size (kb): 7.349999904632568 Features: marker(s): ampR marker(s): kanR promoter: ErmC replicon: pMB1, pUB110 restriction site: EcoRI restriction site: XbaI terminator: fd |
| Applications： | expression vector shuttle vector |
| Comments： | An Escherichia coli/Bacillus subtilis shuttle expression vector utilizing the Erm C promoter of pE194 and an fd terminator. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Grandi G, et al. Plasmid vector containing erythromycin promoter of pE 194 modified by replacement of minus 35 region to increase expression of heterologous protein. Japanese Patent 61,257,189 dated Nov 14 1986 Grandi G, et al. Plasmid vector containing erythromycin promoter of pE 194 modified by replacement of minus 35 region to increase expression of heterologous protein. European Patent 195303 dated Sep 24 1986 Grandi G, et al. Plasmid vector containing erythromycin promoter of pE 194 modified by replacement of minus 35 region to increase expression of heterologous protein. German Federal Republic Patent 3,666,838 dated Dec 14 1989 Guido Grandi, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |