| 产品名称: | pcDNAI/neo-11B2 |
|---|---|
| 商品货号: | TS180602 |
| Designations: | pcDNAI/neo-11B2 |
| Species: | Rattus norvegicus, rat |
| Depositors: | CE Gomez-Sanchez, M Zhou |
| Applications: | in another host, produces protein cytochrome P450, subfamily XIB, polypeptide 2 (steroid-11-beta-hydroxylase, aldosterone synthase) Cytochrome P450, subfamily XIB, polypeptide 2 (aldosterone synthase), aldosterone synthase, steroid 11-beta/18-hydroxylase, steroid-11-beta-hydroxylase, 11-beta-hydroxylase |
| Vector: | Construct size (kb): 8.5 |
| Insert: | DNA: cDNA Insert lengths(kb): 1.5 Gene product: cytochrome P450, subfamily XIB, polypeptide 2 (steroid-11-beta-hydroxylase, aldosterone synthase)( Cytochrome P450, subfamily XIB, polypeptide 2 (aldosterone synthase), aldosterone synthase, steroid 11-beta/18-hydroxylase, steroid-11-beta-hydroxylase, 11-beta-hydroxylase) CYP11B2 Target Gene: cytochrome P450, subfamily XIB, polypeptide 2 (steroid-11-beta-hydroxylase, aldosterone synthase) |
| Insert Size (kb): | 1.5 |
| Media: | ATCC® Medium 1235: LB Medium (ATCC medium 1065) with 5 mcg/ml tetracycline and 10 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): BamHI/XbaI--7.0, 1.6; BamHI/HindIII--7.7, 0.8; HindIII--7.7, 0.8; PvuII--2.1 (doublet), 1.6 (doublet), 0.8, 0.4; XhoI--7.4, 1.1. The insert contains the following restriction sites (nt from the 5 end): XhoI--539; HindIII--726; HincII--948; PvuII--1039. |
| References: | Zhou M, Gomez-Sanchez CE. Cloning and expression of a rat cytochrome P-450 11 beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant. Biochem. Biophys. Res. Commun. 194: 112-117, 1993. PubMed: 8333830 Zimmer WE, et al. Identification of a mouse brain beta-spectrin cDNA and distribution of its mRNA in adult tissues. Brain Res. Bull. 27: 187-193, 1991. PubMed: 1742606 Celso E Gomez-Sanchez, personal communication |