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| 产品名称： | phGK-beta |
|---|---|
| 商品货号： | TS180628 |
| Designations： | phGK-beta |
| GenBank Number： | M88011 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 7.78000020980835 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pSELECT-1 Intact vector size: 5.680 Type of vector: phagemid Vector end: BamHI Vector end: BamHI Cloning sites: Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI PstI SphI HindIII Construction: pBR322, pGEM-3Zf(+) Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): tetR promoter: lac, SP6, T7 replicon: pMB1, f1 Cross references: DNA Seq. Acc.: X65334 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GCK Gene name: glucokinase Contains complete coding sequence?: Y Chromosome: 7; Localization: 7 p Tissue: beta-cell Type of DNA: cDNA Insert end: BamHI Insert end: BamHI Insert size (kb): 2.1 Cross references: DNA Seq. Acc.: M88011 Nucleotides 1-2066 of the insert correspond to nucleotides 30-2095 of M88011. Insert lengths(kb): 2.099999904632568 Tissue: beta-cell Gene product: glucokinase GCK |
| Insert Size (kb)： | 2.100 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.7, 2.1; PvuII--3.1, 2.3, 0.87, 0.8, 0.63, 0.35; HindIII--7.8; BglI--3.7, 2.3, 1.6, 0.2; SphI--7.2, 0.6. Detects genomic EcoRI fragments of the following sizes (kb): human--11.0, 6.1; mouse--18.0. Corresponds to nt 30-2095 of the GenBank record, containing the complete coding sequence (nt 330-1727). |
| References： | Nishi S, et al. Human pancreatic beta-cell glucokinase: cDNA sequence and localization of the polymorphic gene to chromosome 7, band p 13. Diabetologia 35: 743-747, 1992. PubMed: 1511800 Graeme I Bell, personal communication |