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| 产品名称： | Influenza B virus |
|---|---|
| 商品货号： | TS180629 |
| Classification： | Orthomyxoviridae, Influenzavirus B |
| Agent： | Influenza B virus |
| Strain： | B/Virginia/ATCC5/2012 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | This item was isolated in cell culture by three passages in MDCK cells. |
| Effect on Host： | CPE, rounding and sloughing |
| Recommended Host： | MDCK cells (ATCC® CCL-34™)
|
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation:xa03-4 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Source： | Clinical specimen from a patient in Virginia, 2012. |
| References： | WHO Global Influenza Surveillance Network. Manual for the laboratory diagnosis and virological surveillance of influenza. World Health Organization, 2001. IBSN: 9789241548090 |