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| 产品名称： | pMMTV-Sma myc |
|---|---|
| 商品货号： | TS180816 |
| Designations： | pMMTV-Sma myc |
| Species： | Mus musculus, mouse |
| Depositors： | President and Fellows of Harvard College, P Leder, President and Fellows of Harvard College |
| Applications： | A 5.2 kb BamHI(5)/ClaI(3) fragment containing exons 2 and 3 has been used as a probe. Constructed by ligating a SmaI/EcoRI fragment from a genomic subclone of the gene to a SmaI/EcoRI fragment of pA9 (containing the glucocorticoid control region, MMTV promoter and cap site). The EcoRI site used in subcloning was derived from the vector (pBR322). |
| Vector： | Construct size (kb): 14.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pA9 Intact vector size: Type of vector: plasmid Vector end: SmaI Vector end: EcoRI Cloning sites: SmaI EcoRI Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): enhancer: MMTV LTR marker(s): ampR promoter: MMTV LTR Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: mouse Gene symbol: Myc Genomic copy number: unique Gene name: avian myelocytomatosis viral (v-myc) oncogene homolog Contains complete coding sequence?: U Chromosome: 15; Localization: 15 Type of DNA: genomic Insert 5 end: SmaI Insert 3 end: EcoRI Insert size (kb): Cross references: Gene product: avian myelocytomatosis viral (v-myc) oncogene homolog( avian myelocytomatosis viral (v-myc) oncogene homolog, proto-oncogene c-myc) Myc |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--4.5, 4.2, 2.5, 2.0, 0.45; PvuII--5.8, 4.0, 1.75, 1.35, 0.54; EcoRI--14.0; HindIII--7.8, 4.4, 1.15, 0.72; BamHI--9.0, 2.4, 2.0, 1.2; BamHI+HindIII--4.4, 3.8, 2.4, 1.2 (doublet), 0.8; ClaI--9.0, 5.6. There is more than one SmaI site within the myc gene. A 5.2 kb BamHI(5)/ClaI(3) fragment containing exons 2 and 3 has been used as a probe. The BamHI site is in intron 1 and the ClaI site is from the flanking pBR322-derived sequences. This clone is incorrectly cited in U.S. Patent 4,736,866 as ATCC 39746. The insert begins approximately 1.0 kb 5 of exon 1. It therefore contains the 2 promoters naturally preceding the unactivated gene. Constructed by ligating a SmaI/EcoRI fragment from a genomic subclone of the gene to a SmaI/EcoRI fragment of pA9 (containing the glucocorticoid control region, MMTV promoter and cap site). The 3 end of the myc sequence in this construct is the HindIII site approximately 1.0 kb 3 of the poly(A) addition site. The EcoRI site used in subcloning was derived from the vector (pBR322). |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Stewart TA, et al. Spontaneous mammary adenocarcinomas in transgenic mice that carry and express MTV/myc fusion genes. Cell 38: 627-637, 1984 PubMed: 6488314 Leder P, Stewart TA. Transgenic non-human mammals. US Patent 4,736,866 dated Apr 12 1988 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |