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| 产品名称： | pRD6K |
|---|---|
| 商品货号： | TS180839 |
| Designations： | pRD6K |
| Species： | Homo sapiens, human |
| Depositors： | DK Watson |
| Vector： | Construct size (kb): 15.80000019073486 |
| Insert： | DNA: genomic Insert lengths(kb): 5.699999809265137 Tissue: rhabdomyosarcoma RD cell line Gene product: avian erythroblastosis virus E26 (v-ets) oncogene homolog 1 ETS1 Alleles: A1, A2, B1, B2, C1, C2, E1, E2, F2, F1 |
| Insert Size (kb)： | 5.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.7 (doublet), 4.4; HindIII--5.6, 5.0, 3.8, 0.75 (doublet); EcoRI/HindIII--4.4, 3.8 (doublet), 1.2 (doublet), 0.75 (doublet); BamHI--5.4, 5.0, 4.2, 0.65 (doublet); PvuII--7.8, 5.8, 2.6. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--0.7, 5.0. Detects a human genomic EcoRI fragment of 6.2 kb, The 0.7 kb HindIII/EcoRI fragment contains coding sequences. and a mRNA of 6.8 kb in HeLa cells. The plasmid contains two copies of the insert. |
| References： | Watson DK, et al. The ets sequence from the transforming gene of avian erythroblastosis virus, E26, has unique domains on human chromosomes 11 and 21: both loci are transcriptionally active. Proc. Natl. Acad. Sci. USA 82: 7294-7298, 1985. PubMed: 2997781 Watson DK, et al. Conserved chromosomal positions of dual domains of the ets protooncogene in cats, mice, and humans. Proc. Natl. Acad. Sci. USA 83: 1792-1796, 1986. PubMed: 3513188 Dennis K Watson, personal communication |