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微信小章| 产品名称： | pFL44S |
|---|---|
| 商品货号： | TS180910 |
| Designations： | pFL44S |
| Depositors： | F Lacroute |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.3189997673034670 Vector: pFL44S (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):URA3,ampR Construct size (kb): 4.318999767303467 Features: insert detection: lacZ marker(s): ampR, URA3 promoter: lac replicon: pMB1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector shuttle vector vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.4; BglII--3.3, 1.1; ClaI--3.9, 0.5. The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 13.4. YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. The order of the major features in this plasmid is: EcoRI/MCS/HindIII - URA3 - 2 micron ori - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470 Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589 Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827 Chevallier MR, et al. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene 11: 11-19, 1980. PubMed: 7002730 |
| Shipped： | freeze-dried |