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| 产品名称： | p58-CL42 |
|---|---|
| 商品货号： | TS180916 |
| Designations： | p58-CL42 |
| GenBank Number： | U24076 |
| Species： | Homo sapiens, human |
| Depositors： | N Wagtmann |
| Vector： | Construct size (kb): 5.699999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pSPORT1 Intact vector size: 4.109 Type of vector: plasmid Vector end: SalI Vector end: NotI Cloning sites: AatII SphI mLuI SunI SnaBI HindIII BamHI XbaI XmaIII NotI SpeI SstI SalI AccI SmaI EcoRI Kpn2I RsrII PinAI KpnI Sse8387I PstI Polylinker sites: AatII SphI mLuI SunI SnaBI HindIII BamHI XbaI XmaIII NotI SpeI SstI SalI AccI SmaI EcoRI Kpn2I RsrII PinAI KpnI Sse8387I PstI Host range: Escherichia coli Features (with orientation and position when available): promoter for in vitro transcription: SP6, ->, 164-180 MCS: AatII...PstI, ->, 191-285 promoter for in vitro transcription: T7, insert detection: lacZ, repressor gene: lacI, replicon: pMB1, 1903-1903 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: KIR Other gene symbols: NKB1 Gene name: killer cell inhibitory receptor ORFs seq. position: GDB probe: GDB:698358 () Contains complete coding sequence?: Y Chromosome: 19; Localization: 19 q13.4; 19, 374.860000 cR Tissue: Resting CD3- cells Type of DNA: cDNA Insert 5 end: Modification: SalI linker Insert 3 end: Modification: NotI linker Insert size (kb): 1.608 Cross references: DNA Seq. Acc.: U24076 Nucleotides 1-1593 of the insert correspond to nucleotides 1-1593 of U24076. Insert lengths(kb): 1.608000040054321 Tissue: Resting CD3- cells Gene product: killer cell inhibitory receptor KIR |
| Insert Size (kb)： | 1.608 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): NotI/SalI--4.4, 1.6; BglI--3.9, 1.3, 0.8; EcoRV--4.5, 1.5; PstI--5.0, 0.4, 0.35, 0.1; PvuII--3.7, 1.4, 0.6. The insert contains the following restriction sites (approximate kb from the 5 end): BclI--0.40; BglI--1.09; ClaI--1.20; EcoRV--1.01; NcoI--1.59; PstI--0.08, 0.48, 0.55, 0.87; PvuII--1.16; SacI--0.49; StuI--1.09. Encodes a variant recognized by monoclonal antibody EB6 but not GL183. |
| References： | Wagtmann N, et al. Molecular clones of the p58NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains. Immunity 2: 439-449, 1995. PubMed: 7749980 |