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| 产品名称： | pK19mob |
|---|---|
| 商品货号： | TS180920 |
| Designations： | pK19mob |
| Depositors： | A Schaefer |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli S17-1 |
| Vector Information： | Size (kb): 3.7599999904632570 DESCRIPTION OF VECTOR: Intact vector size: 3.760 Type of vector: plasmid Cloning sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI EcoRI Polylinker sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI EcoRI Construction: pK19, pSUP102 (RP4 mob) Host range: Escherichia coli; Salmonella sp.; Serratia sp. Features (with orientation and position when available): marker(s): kanR, -> replicon: oriT replicon: oriV insert detection: lacZ, -> MCS: HindIII...EcoRI, -> Vector: pK19mob (plasmid) Construction: pK19, pSUP102 (RP4 mob) Marker(s):kanR Construct size (kb): 3.759999990463257 Features: insert detection: lacZ marker(s): kanR replicon: oriT replicon: oriV MCS: HindIII...EcoRI |
| Applications： | integrating vector mobilizable vector vector containing primer sites useful for sequencing vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.8; HindIII--3.8; PstI--3.8. After mobilization, the plasmid can be maintained by integration into the host chromosome via homologous recombination. Differs from pK18mob (ATCC 87095) only in the orientation of the polylinker. Cloning vector allowing mobilization into a wide range of Gram- and Gram+ bacteria. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983. Schafer A, et al. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145: 69-73, 1994. PubMed: 8045426 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |