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微信小章| 产品名称： | c8035 8035 |
|---|---|
| 商品货号： | TS180963 |
| Designations： | c8035 8035 |
| GenBank Number： | U33050 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | MV Olson, L Riles |
| Applications： | The ATCC encourages users of this material to communicate their results to us. |
| Vector： | Construct size (kb): 42.58 |
| Insert： | DNA: genomic Insert information: Insert size (kb): xa036.146 Insert endsxa0: MboI Genesxa0: xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 mating factor a, MFA1, xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 ribosomal protein L27b, RPL27B xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 negative regulator of prp genes,xa0 SPP41 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 nuclear-localized protein containing zinc finger xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 motifs, involved in pre-tRNA splicing, STP1 DNAxa0: genomic, chromosome IV R Sourcexa0: Saccharomyces cerevisiaexa0AB972 (ATCC 76269) Genbank accessionxa0: Nucleotides 1-36146 of the insert correspond to nucleotides 2618-38763 of U33050. Insert lengths(kb): 36.14599990844727 Gene product: mating factor a MFA1 Gene product:ribosomal protein L27b (RPL27B) Gene product: negative regulator of prp genes (SPP41) Gene product: nuclear-localized protein containing zinc finger motifs, involved in pre-tRNA splicing (STP1) |
| Insert Size (kb)： | 36.146 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | component of:ATCC 77284 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Frozen glycerol stock of E. coli containing the cosmid. |
| Comments： | Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 3.077, 2.207, 2.564, 2.534, 0.728, 1.488, 2.242, 0.862, 3.562, 2.528, 2.803, 0.799, 1.821, 2.002, 1.215, 0.545, 1.168, 0.406. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70439, 70457, 70572, 70736. NOTE: Because of the large number of simultaneous deposits from Dr. Olson, the ATCC has not verified that the characteristics of the material supplied here are as published. The ATCC encourages users of this material to communicate their results to us. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |