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| 产品名称： | T12a D7S2742, UTR-9783 |
|---|---|
| 商品货号： | TS180967 |
| Designations： | T12a D7S2742, UTR-9783 |
| GenBank Number： | M28668 |
| Species： | Homo sapiens, human |
| Depositors： | JR Riordan |
| Vector： | Construct size (kb): 6.050000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.099999904632568 Tissue: colon carcinoma T84 cell line Gene product: cystic fibrosis transmembrane conductance regulator CF Alleles: A1, A1, A1, A1, A2, B1, B2, A1, A1, A2, B1, B2, A2, A2, A2, A2 |
| Insert Size (kb)： | 3.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Contains an internal EcoRI site. An EcoRI digest of the plasmid results in fragments of 2.3 and 0.8 kb from the insert. Restriction digests of the clone give the following sizes (kb): XhoI/HindIII--3.7, 0.87, 0.8, 0.5; HindIII--3.7, 0.87, 0.8, 0.5; EcoRI--2.8, 2.4, 0.85; BamHI--3.9, 1.8, 0.2. This clone includes exons 14 - 24 and hybridizes to genomic EcoRI fragments of 1.8, 3.5, 1.9, 4.5, 2.4, 1.0, 2.8, 1.8, 8.5, 2.2, and 2.3 kb. |
| References： | Riordan JR, et al. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245: 1066-1073, 1989. PubMed: 2475911 J R Riordan, personal communication |