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| 产品名称： | p423 GALL |
|---|---|
| 商品货号： | TS181141 |
| Designations： | p423 GALL |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.4590001106262210 DESCRIPTION OF VECTOR: Intact vector size: 6.459 Type of vector: plasmid Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI Construction: pRS423, GALL promoter Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): marker(s): HIS3, ->, 200-1395 terminator: CYC1, ->, 2077-2337 MCS: XhoI...SpeI, ->, 2337-2394 promoter for expression: GALL, marker(s): ampR, replicon: 2 micron, ->, 5012-6352 Vector: p423 GALL (plasmid) Promoters: Promoter for expression GALL Construction: pRS423, GALL promoter Marker(s):ampR,HIS3 Construct size (kb): 6.459000110626221 Features: marker(s): HIS3 marker(s): ampR promoter for expression: GALL replicon: 2 micron MCS: XhoI...SpeI terminator: CYC1 |
| Applications： | YE-type (episomal) shuttle vector YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.45; EcoRI--6.5; KpnI--5.5, 1.0. The galactokinase (GALL) promoter is a deletion variant of GAL1, which lacks one of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose and is induced at a moderate level by galactose. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736 |
| Related Products： | component of:ATCC 87670 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |