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| 产品名称： | pRS413 |
|---|---|
| 商品货号： | TS181366 |
| Designations： | pRS413 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH5alpha |
| Vector Information： | Size (kb): 4.97 DESCRIPTION OF VECTOR: Intact vector size: 4.970 Type of vector: phagemid Cloning sites: ApaI XhoI SalI ClaI EcoRV EcoRI SmaI BamHI SpeI XbaI EagI NotI SacII SacI NaeI Polylinker sites: BssHII KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI BstXI SacII SacI BssHII Construction: pBluescriptII KS+ Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): marker(s): HIS3, ->, 504-1163 replicon: f1, insert detection: lacZ, promoter for in vitro transcription: T7, ->, 2049-2068 MCS: KpnI...SacI, ->, 2076-2183 promoter for in vitro transcription: T3, promoter: lac, replicon: pMB1, 2638-2638 marker(s): ampR, centromere: CEN6, 4398-4514 replicon: ARSH4, 4512-4885 Vector: pRS413 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pBluescriptII KS+ Marker(s):ampR,HIS3 Construct size (kb): 4.97 Features: insert detection: lacZ marker(s): HIS3 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARSH4 replicon: f1 replicon: pMB1 MCS: KpnI...SacI centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuII--4.55, 0.45; BglI--2.3, 1.7, 1.0; PstI--4.05, 0.95. A YC-type shuttle vector with HIS3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 |
| Related Products： | component of:ATCC 87531 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |