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| 产品名称： | pG95/T1 |
|---|---|
| 商品货号： | TS181531 |
| Designations： | pG95/T1 |
| GenBank Number： | X56215 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff |
| Applications： | in another host, produces protein mitochondrial protein, 43 kDa, required for expression of subunit 9 of F0 ATPase (oli1) |
| Vector： | Construct size (kb): 13.80000019073486 |
| Insert： | DNA: genomic Insert lengths(kb): 6.0 Gene product: mitochondrial protein, 43 kDa, required for expression of subunit 9 of F0 ATPase (oli1) ATP13 Target Gene: mitochondrial protein, 43 kDa, required for expression of subunit 9 of F0 ATPase (oli1) |
| Insert Size (kb)： | 6.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.6, 3.7, 2.3, 1.0, 0.6; SstI--12.0, 1.7; SmaI/XhoI--9.4, 4.6; Xba--5.2, 4.8, 4.4; SalI/SmaI--7.0 (doublet). The insert contains the following restriction sites (approximate kb from the end closer to the SalI site of the vector nt of published sequence): XbaI--0.5, 4.5; HindIII--1.3 1, 2.3 1004, 2.5 1269; XhoI--1.9 656; SstI--0.6, 2.25 969. EcoRI--2.5 1227, 3.2, 4.1. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Ackerman SH, et al. ATP13, a nuclear gene of Saccharomyces cerevisiae essential for the expression of subunit 9 of the mitochondrial ATPase. FEBS Lett. 278: 234-238, 1991. PubMed: 1825065 |