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| 产品名称： | pCP2-4-10 |
|---|---|
| 商品货号： | TS181542 |
| Designations： | pCP2-4-10 |
| Depositors： | E.I. du Pont, SC Falco, E.I. du Pont |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 |
| Vector Information： | Size (kb): 0E-16 DESCRIPTION OF VECTOR COMPONENT: Name of vector: unknown Intact vector size: Type of vector: plasmid Vector end: ClaI Vector end: HindIII Cloning sites: Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR Cross references: Vector: pCP2-4-10 (plasmid) Construction: YEp24, Saccharomyces cerevisiae ILV2 Marker(s):URA3,ampR,ILV2 Construct size (kb): 0.0 Features: marker(s): ILV2 marker(s): URA3 marker(s): ampR replicon: 2 micron replicon: pMB1 |
| Applications： | YE-type (episomal) shuttle vector contains sequence acetohydroxyacid synthase shuttle vector |
| Comments： | An episomal shuttle vector with dominant selectable marker for resistance in Saccharomyces cerevisiae to sulfometuron methyl, a sulfonylurea herbicide. The ILV2 fragment can be excised as a ClaI/HindIII fragment. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Falco SC. Selectable markers for yeast transformation. US Patent 4,626,505 dated Dec 2 1986 Sauer BL. Site-specific recombination of DNA in eukaryotic cells. US Patent 4,959,317 dated Sep 25 1990 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |