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| 产品名称： | Human respiratory syncytial virus |
|---|---|
| 商品货号： | TS181893 |
| Classification： | Paramyxoviridae, Pneumovirus |
| Deposited As： | Respiratory syncytial virus |
| Agent： | Human respiratory syncytial virus |
| Strain： | 18537 |
| Applications： | Respiratory research |
| Positive for Mycoplasma： | No |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | The early passage history of this item is unknown. |
| Effect on Host： | CPE, massive syncytia formation and sloughing |
| Recommended Host： | HEp-2 (ATCC® CCL-23x99) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 3-7 days |
| Name of Depositor： | RM Chanock, J Crowe, National Institute of Allergy and Infectious Diseases; ATCC® |
| Special Collection： | ATCC |
| Source： | Respiratory secretions from child with acute respiratory disease seen at Children’s Hospital of the District of Columbia, Washington, DC, 1962. Treated ATCC® VR-1401™ to remove mycoplasma at ATCC®. |
| References： | Coates HV, et al. An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. Am. J. Epidemiol. 83: 299-313, 1966. PubMed: 5933417 |