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| 产品名称： | pCD11B |
|---|---|
| 商品货号： | TS181973 |
| Designations： | pCD11B |
| GenBank Number： | M19503 |
| Species： | Homo sapiens, human |
| Depositors： | MF Singer, RE Thayer |
| Vector： | Construct size (kb): 5.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.585000038146973 Tissue: teratocarcinoma NTera2D1 cell line Gene product: LINE consensus sequence (KpnI sequence), repetitive |
| Insert Size (kb)： | 2.585 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.6; KpnI--4.1, 1.2; HindIII--5.0, 0.3; PstI--5.3; BglI--4.1, 1.2. The insert contains the following restriction sites (nt position according to the published sequence): KpnI--3423, 4627; HindIII--3624. Contains nt 3396-5975 of the reported sequence, including the 3 end of ORF2 (nt 1929-5781). |
| References： | Skowronski J, et al. Unit-length Line-1 transcripts in human teratocarcinoma cells. Mol. Cell. Biol. 8: 1385-1397, 1988. PubMed: 2454389 |