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| 产品名称： | pUD18 |
|---|---|
| 商品货号： | TS181990 |
| Designations： | pUD18 |
| Species： | Schizosaccharomyces pombe Lindner, fission yeast |
| Depositors： | NC Barbet, AM Carr |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 1.700000047683716 Gene product: dihydroorotase ura4 |
| Insert Size (kb)： | 1.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.4; NotI--4.4; EcoRV--3.2, 1.2; PstI--4.4; EcoRV/NotI--2.6, 1.2, 0.6. Donor plasmid for gene disruption experiments in S. pombe. The AccIII ends can be partially filled in with dCTP using T4 DNA polymerase, and ligated to NotI ends partially filled in with dGTP. The vector was derived from pUC18 by replacing the HindIII/EcoRI MCS with an adapter providing a MCS with the following sites: AccIII EcoRV SpeI NotI AccIII. |
| References： | Barbet N, et al. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114: 59-66, 1992. PubMed: 1587485 |