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| 产品名称： | pGEX-PEA3 pGEX.PEA3.1 |
|---|---|
| 商品货号： | TS182028 |
| Designations： | pGEX-PEA3 pGEX.PEA3.1 |
| GenBank Number： | X63190 |
| Species： | Mus musculus, mouse |
| Depositors： | JA Hassell |
| Applications： | produces protein polyomavirus enhancer activator 3 |
| Vector： | Construct size (kb): 6.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.600000023841858 Tissue: FM3A cell line Gene product: polyomavirus enhancer activator 3 PEA3 Target Gene: polyomavirus enhancer activator 3 |
| Insert Size (kb)： | 1.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.9, 1.6; PstI--6.5; SmaI--6.5. The insert contains the following restriction sites (nt from the 5 end): PvuII--1138, 1492; SacI--1103. The glutathione-S-transferase fusion protein product of this construct demonstrates DNA-binding activity. A partial cDNA clone beginning at nt 803 of the published sequence. Detects mRNA of 2.4 kb and 4.1 kb. The smaller species is predominant in murine cells, and the larger species is predominant in human and monkey cells. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Xin JH, et al. Molecular cloning and characterization of PEA3, a new member of the Ets oncogene family that is differentially expressed in mouse embryonic cells. Genes Dev. 6: 481-496, 1992. PubMed: 1547944 John A Hassell, personal communication |