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| 产品名称： | pGS14-10 |
|---|---|
| 商品货号： | TS182032 |
| Designations： | pGS14-10 |
| Species： | Homo sapiens, human |
| Applications： | A 260 bp AluI/PstI fragment in intron 3 has been shown to be single copy and useful for mapping purposes. |
| Vector： | Construct size (kb): 12.39999961853027 |
| Insert： | DNA: genomic Insert lengths(kb): 10.0 Tissue: HeLa cell line Gene product: ribosomal protein S14 RPS14 |
| Insert Size (kb)： | 10.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--9.8, 2.8; EcoRI--3.8, 3.2, 3.0, 2.3; PstI--4.8, 3.2, 2.3, 1.8; SalI--13.0; BamHI--4.6, 4.1, 3.8. The insert extends from an EcoRI site to the HindIII site (about 1.5 to 11.5 kb). It includes 3 copies of a 10 bp sequence (-69, -29, +1) found in other ribosomal protein genes. The coding sequence starts in exon 2. Introns 3 and 4 contain Alu-homologous repeats. The 3 flanking sequence contains 10 bp complementary to U1 RNA. A 260 bp AluI/PstI fragment in intron 3 has been shown to be single copy and useful for mapping purposes. It shows only slight cross-reaction with CHO cell (Chinese hamster) DNA. The fragment cloned was an EcoRI/HindIII fragment to which a HindIII linker was added. Thus the clone contains both EcoRI and HindIII sites at its 5 end and a HindIII site at the 3 end. There are several internal EcoRI sites. |
| References： | Diaz JJ, Roufa DJ. Fine-structure map of the human ribosomal protein gene RPS14. Mol. Cell. Biol. 12: 1680-1686, 1992. PubMed: 1549121 Rhoads DD, et al. Primary structure of human ribosomal protein S14 and the gene that encodes it. Mol. Cell. Biol. 6: 2774-2783, 1986. PubMed: 3785212 |