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购买须知
| 产品名称： | 22/1:2.1 p22/1.2.1, p22/1:2.1 |
|---|---|
| 商品货号： | TS182047 |
| Designations： | 22/1:2.1 p22/1.2.1, p22/1:2.1 |
| Depositors： | H McDermid |
| Applications： | At low stringency (2X SSC, 50% formamide, 37C), silver grains are observed over centromeric regions of a number of chromosomes. |
| Vector： | Construct size (kb): 4.800 |
| Insert： | DNA: genomic
Insert lengths(kb): 2.100
Tissue: lymphoblast GM1416 (48,XXXX) cell line
Gene product: DNA Segment, repetitive D22Z2 |
| Insert Size (kb)： | 2.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Plasmid in Escherichia coli |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.1; HindIII--3.5, 1.3; BamHI--4.8; PstI--4.8; SalI--4.8. A 0.73 kb EcoRI/HindIII fragment from the insert has been sequenced partially, demonstrating a repeat of about 171 bp with homology to alpha satellite sequence families. At low stringency (2X SSC, 50% formamide, 37C), silver grains are observed over centromeric regions of a number of chromosomes. At high stringency (0.2X SSC, 50% formamide, 37C), the signal is predominately at the 22 centromeric region. |
| References： | McDermid HE, et al. Isolation and characterization of an alpha-satellite repeated sequence from human chromosome 22. Chromosoma 94: 228-234, 1986. PubMed: 3769652 |