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| 产品名称： | pBLCAT6 |
|---|---|
| 商品货号： | TS182082 |
| Designations： | pBLCAT6 |
| Depositors： | M Boshart |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.2500000000000000 Vector: pBLCAT6 (plasmid) Promoters: Promoter none Construction: pBLCAT3 Marker(s):ampR Construct size (kb): 4.25 Features: marker(s): ampR promoter: none replicon: pMB1 terminator: SV40 polyadenylation |
| Applications： | promoter-cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.6; PvuI--4.3; PvuII--2.5, 1.8; BglI/XhoI--2.9, 1.4; ClaI/XhoI--4.3. The vector contains the following restriction sites (approximate kb from nt 1): BglI--3.07; ClaI--1.69; EcoRI--0.30, 1.71; HindIII--0.0; NcoI--0.60; PvuI--3.33; PvuII--0.20, 1.89; SacI--1.70; XhoI--0.05. Promoter-cloning vector for analysis of promoters in vertebrate cells, using the CAT reporter gene. Constructed from pBLCAT3 (ATCC 37528) by deletion of a 467 bp fragment immediately upstream from the 5 polylinker and replacement by two fragments, each containing the polyadenylation signal of the SV40 large T gene. The order of the major features in the plasmid is: two SV40 polyadenylation signals - HindIII/MCS/XhoI - cat - SV40 small T splice site - SV40 polyadenylation signal - ClaI/MCS/SstI - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Boshart M, et al. Reporter constructs with low background activity utilizing the cat gene. Gene 110: 129-130, 1992. PubMed: 1544570 |
| Shipped： | freeze-dried |