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微信小章| 产品名称： | pGEM-3.110B-1 pAS-h-IgER-110B, pGEM-3-110B-1, pXS |
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| 商品货号： | TS182191 |
| Designations： | pGEM-3.110B-1 pAS-h-IgER-110B, pGEM-3-110B-1, pXS |
| GenBank Number： | J03605 |
| Species： | Homo sapiens, human |
| Depositors： | P Leder |
| Applications： | pGEM-3.110B-1 has been deposited in the NIH repository (TS182191) and to support a patent application (ATCC 67566). The probe used to detect the RFLP was a 0.9 kb XbaI/SfaN1 fragment which includes the entire reading frame but excludes some of the 3 sequences. The entire cDNA was also used. |
| Vector： | Construct size (kb): 4.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.100000023841858 Tissue: mast cell KU812 cell line Gene product: Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide( Fc fragment of Ige, high affinity I, receptor for, alpha polypeptide, immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide, Fc IgE receptor, alpha polypeptide) FCE1A Alleles: A2, A2, A1, A1 |
| Insert Size (kb)： | 1.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI/SacI--2.9, 1.1; EcoRI--4.0; HindIII--4.0; XbaI--4.0; SacI--4.0. pGEM-3.110B-1 has been deposited in the NIH repository (TS182191) and to support a patent application (ATCC 67566). This clone encodes a complete alpha chain and includes 5 and 3 flanking sequences with a poly(A) tail. The XbaI site is at the 5 end. The probe used to detect the RFLP was a 0.9 kb XbaI/SfaN1 fragment which includes the entire reading frame but excludes some of the 3 sequences. The entire cDNA was also used. The probe detects human EcoRI fragments of 4.4, 3.0, and 1.5 kb and mouse EcoRI fragments of 4.9, 3.9, and 1.7 kb. Enzyme(s) not detecting polymorphism: AccI, AvaII, BamHI, BanI, BglII, DdeI, EcoRI, EcoRV, HincII, HindIII, HinfI, MspI, PstI, PvuII, SspI, TaqI, XbaI. |
| References： | Tepler I, et al. The gene for the human mast cell high-affinity IgE receptor alpha chain: chromosomal localization to 1q21-q23 and RFLP analysis. Am. J. Hum. Genet. 45: 761-765, 1989. PubMed: 2573277 Shimizu A, et al. Human and rat mast cell high-affinity immunoglobulin E receptors: characterization of putative alpha-chain gene products. Proc. Natl. Acad. Sci. USA 85: 1907-1911, 1988. PubMed: 2964640 Leder P, et al. DNA encoding IGE receptor alpha-subunit or fragment thereof. US Patent 4,962,035 dated Oct 9 1990 |