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| 产品名称： | pHLIFR-65 |
|---|---|
| 商品货号： | TS182199 |
| Designations： | pHLIFR-65 |
| GenBank Number： | X61615 |
| Species： | Homo sapiens, human |
| Depositors： | Immunex Corporation, M Beckmann, Immunex Corporation |
| Applications： | in another host, produces protein leukemia inhibitory factor receptor |
| Vector： | Construct size (kb): 8.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.200000047683716 Tissue: placenta Gene product: leukemia inhibitory factor receptor LIFR Target Gene: leukemia inhibitory factor receptor |
| Insert Size (kb)： | 3.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--5.0, 2.7, 0.62; PstI--3.3, 2.7, 2.4; PvuII--6.5, 1.7. The insert contains the following restriction sites (approximate kb from the 5 end): BglII--0.0, 0.6; EcoRV--1.2, 2.3; PstI--0.1, 2.4; PvuII--1.5; SacI--0.7. A partial cDNA clone corresponding to nt 1-3138 of the reported sequence (complete coding region 179-3472). The truncated product still confers LIF binding activity. Detects transcripts of approximately 6 kb, 4.5 kb and 5 kb (minor) in human placenta. pDC302 is a mammalian expression vector containing promoter and enhancer sequences from SV40 and CMV, leader sequences from adenovirus 2 followed by a multiple cloning site, SV40 polyadenylation and termination signals and adenovirus genes VAI and VAII. Digestion with BglII yields a 5 kb vector fragment and fragments of 2.6 kb and 0.60 kb from the insert. |
| References： | Gearing DP, et al. Leukemia inhibitory factor receptor is stucturally related to the IL-6 signal tranducer, gp130. EMBO J. 10: 2839-2848, 1991. PubMed: 1915266 Gearing DP. Receptor for oncostatin M and leukemia inhibitory factor. US Patent 5,262,522 dated Nov 16 1993 M Patricia Beckmann, personal communication |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |