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微信小章| 产品名称： | pAIIe/6 |
|---|---|
| 商品货号： | TS182304 |
| Designations： | pAIIe/6 |
| GenBank Number： | J04695 |
| Species： | Mus musculus, mouse |
| Depositors： | I Oberbaumer |
| Applications： | The internal 0.8 StuI fragment is recommended for hybridization. |
| Vector： | Construct size (kb): 3.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.8799999952316284 Tissue: teratocarcinoma PYS-2/12 cell line Gene product: collagen, type IV, alpha 2 Col4a2 |
| Insert Size (kb)： | 0.880 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.72, 0.88; EcoRI--3.6; BamHI--3.5, 0.1; PstI--3.6; SalI--3.6. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.01; KpnI--0.02; StuI--0.03, 0.87; PvuII--0.07; SmaI--0.23; BglI--0.34, 0.66. Extends from nt 2331 to 3216 of the reported sequence, encoding part of the triple helical domain. The internal 0.8 StuI fragment is recommended for hybridization. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975 Saus J, et al. The complete primary structure of mouse alpha2(IV) collagen. J. Biol. Chem. 264: 6318-6324, 1989. PubMed: 2703491 Brazel D, et al. Human basement membrane collagen (type IV)-- The amino acid sequence of the alpha2(IV) chain and its comparison with the alpha1(IV) chain reveals deletions in the alpha1(IV) chain. Eur. J. Biochem. 172: 35-42, 1988. PubMed: 3345760 Ilse Oberbaumer, personal communication |