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微信小章| 产品名称： | Paramecium primaurelia Sonneborn |
|---|---|
| 商品货号： | TS182328 |
| Strain Designations： | stock 258 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Mexico, 1956 |
| Product Format： | test tube |
| Type Strain： | no |
| Medium： | ATCC® Medium 802: Sonneborns Paramecium medium |
| Growth Conditions： | Max Temperature: 31.0°C Min Temperature: 13.0°C Protocol: ATCCNO: 30300 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, and loosen the cap one half turn. Add 1.0 ml of ATCC medium 802 bacterized with Enterobacter aerogenes ATCC 13048 twice weekly. When the tube is filled to within one inch of the top, decant leaving 5.0 ml in the original tube. Subcultures are established by transferring 0.5 ml of a growing culture to 5.0 ml of bacterized ATCC medium 802 in a 20 x 120 mm test tube. |
| Subcultivation： | Protocol: ATCCNO: 30300 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, and loosen the cap one half turn. Add 1.0 ml of ATCC medium 802 bacterized with Enterobacter aerogenes ATCC 13048 twice weekly. When the tube is filled to within one inch of the top, decant leaving 5.0 ml in the original tube. Subcultures are established by transferring 0.5 ml of a growing culture to 5.0 ml of bacterized ATCC medium 802 in a 20 x 120 mm test tube. |
| Cryopreservation： | Cryoprotective Solution DMSO xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 1.5 ml Fresh growth medium w/o bacteriaxa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 7.5 ml MgCl2 (0.5 mM)xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 0.5 ml CaCl2 (0.5 mM)xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 0.5 ml 1.xa0xa0 Mix the components in the order listed.xa0 Before adding the MgCl2 and the CaCl2 allow the solution to return to room temperature.xa0 When the medium is added to the DMSO the solution will warm up due to chemical heat. 2. xa0 Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 200 x g for 1 min. 3. Adjust the concentration of cells to 2 x 105/ml in fresh medium. 4.xa0 Mix the cell preparation and the cryoprotective solution in equal portions. 5.xa0 Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 6.xa0 Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. 7.xa0 Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator. 8. To establish a culture from the frozen state add 1.0 ml ATCC medium 802 to the frozen ampule and place it in a 35°C water bath.xa0 Immerse the vialxa0 to a level just above the surface of the frozen material. Do not agitate the vial. 9.xa0xa0 Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate onto the surface of an ATCC medium 919 (non-nutrient agar) plate containing an overlay of 15.0 ml of bacterized ATCC medium 802. 10. Incubate at 25°C. 11. Once the culture is established, transfer 0.5 ml to 5.0 ml of bacterized ATCC medium 802. 12. Follow the protocol for maintenance of culture. |
| Name of Depositor： | TM Sonneborn |
| Year of Origin： | 1956 |
| Cross References： | Nucleotide (GenBank) : U00597 Herpetomonas megaseliae kinetoplast clone 1.1 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00613 Herpetomonas megaseliae kinetoplast clone 1.4 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00618 Herpetomonas megaseliae kinetoplast clone 1.5 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00627 Herpetomonas megaseliae kinetoplast clone 1.6 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00632 Herpetomonas megaseliae kinetoplast clone 1.7 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00634 Herpetomonas megaseliae kinetoplast clone 1.8 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00598 Herpetomonas megaseliae kinetoplast clone 1.10 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00600 Herpetomonas megaseliae kinetoplast clone 1.13 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00601 Herpetomonas megaseliae kinetoplast clone 1.14 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00602 Herpetomonas megaseliae kinetoplast clone 1.16 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00603 Herpetomonas megaseliae kinetoplast clone 1.17 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00604 Herpetomonas megaseliae kinetoplast clone 1.19 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00605 Herpetomonas megaseliae kinetoplast clone 1.21 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00606 Herpetomonas megaseliae kinetoplast clone 1.30 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00607 Herpetomonas megaseliae kinetoplast clone 1.31 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00608 Herpetomonas megaseliae kinetoplast clone 1.32 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00609 Herpetomonas megaseliae kinetoplast clone 1.37 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00610 Herpetomonas megaseliae kinetoplast clone 1.36 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00611 Herpetomonas megaseliae TS182328 kinetoplast partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00612 Herpetomonas megaseliae kinetoplast clone 1.39 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00614 Herpetomonas megaseliae kinetoplast clone 1.43 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00615 Herpetomonas megaseliae kinetoplast clone 1.44 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00616 Herpetomonas megaseliae kinetoplast clone 1.46 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00617 Herpetomonas megaseliae kinetoplast clone 1.48 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00622 Herpetomonas megaseliae kinetoplast clone 1.51 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00623 Herpetomonas megaseliae kinetoplast clone 1.52 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00624 Herpetomonas megaseliae kinetoplast clone 1.53 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00625 Herpetomonas megaseliae kinetoplast clone 1.57 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00626 Herpetomonas megaseliae kinetoplast clone 1.59 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00628 Herpetomonas megaseliae kinetoplast clone 1.60 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00629 Herpetomonas megaseliae kinetoplast clone 1.61 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00630 Herpetomonas megaseliae kinetoplast clone 1.64 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00631 Herpetomonas megaseliae kinetoplast clone 1.68 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00633 Herpetomonas megaseliae kinetoplast clone 1.70 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00661 Herpetomonas megaseliae kinetoplast clone 1.20 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. Nucleotide (GenBank) : U00599 Herpetomonas megaseliae kinetoplast clone 1.100 partially edited cytochrome oxidase subunit III (COIII) RNA sequence. |