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| 产品名称： | Galpha z-WT pBN36 |
|---|---|
| 商品货号： | TS182402 |
| Designations： | Galpha z-WT pBN36 |
| GenBank Number： | J03773 |
| Species： | Rattus norvegicus, rat |
| Depositors： | P Herzmark, HR Bourne |
| Applications： | in another host, produces protein guanine nucleotide binding protein (G protein), alpha z polypeptide guanine nucleotide-binding protein, alpha z polypeptide |
| Vector： | Construct size (kb): 5.53000020980835 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.529000043869019 Gene product: guanine nucleotide binding protein (G protein), alpha z polypeptide(guanine nucleotide-binding protein, alpha z polypeptide) GNAZ Target Gene: guanine nucleotide binding protein (G protein), alpha z polypeptide |
| Insert Size (kb)： | 1.529 |
| Media： | ATCC® Medium 1890: LB medium (ATCC medium 1065) with 12 mcg/ml tetracycline and 100 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.8, 0.75; BglI--1.75 (doublet), 1.7, 0.3 + smaller; EcoRI--4.0, 1.55; HindIII--4.7, 0,85; XbaI--5.6. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.65; BglI--1.22, 1.49; BglII--0.97; HindIII--0.71; NcoI--0.01; PvuII--1.52; SacI--0.69; SmaI--0.42; StuI--0.20, 0.43. Deposited with the understanding that the DNAs not be used in human experiments. Encodes a hemagglutinin epitope replacing nt 409-426 of the GenBank sequence. |
| References： | Wong YH, et al. Gz-mediated hormonal inhibition of cyclic AMP accumulation. Science 255: 339-342, 1992. PubMed: 1347957 Paul Herzmark, personal communication |