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| 产品名称： | YIp33 pRB19 |
|---|---|
| 商品货号： | TS182465 |
| Designations： | YIp33 pRB19 |
| Depositors： | D Botstein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.6999998092651370 Vector: YIp33 (plasmid) Construction: pBR322, pYe(Leu2)10 Marker(s):LEU2,ampR Construct size (kb): 6.699999809265137 Features: marker(s): ampR, LEU2 replicon: pMB1 |
| Applications： | YI-type (integrating) shuttle vector integrating vector shuttle vector |
| Comments： | A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector. This was constructed by inserting a 2.2 kb SalI/XhoI fragment containing LEU2 from pYe(Leu2)10 into the SalI site of pBR322. The 5 - 3 orientation of LEU2 is counterclockwise. LEU2, originating from strain X2180-1A, includes a region of Ty1-17. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030 Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863 |
| Related Products： | component of:ATCC 77188 |
| Shipped： | freeze-dried |