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| 产品名称： | pCE54 |
|---|---|
| 商品货号： | TS182538 |
| Designations： | pCE54 |
| Depositors： | Kyowa Ferm. Ind. Co., Ltd., S Wakaki, Kyowa Ferm. Ind. Co., Ltd. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Corynebacterium glutamicum LA103 |
| Vector Information： | Size (kb): 14.5000000000000000 Vector: pCE54 (plasmid) Construction: pCG2, pGA22 Marker(s):cmlR,kanR,tetR Construct size (kb): 14.5 Features: marker(s): tetR, cmlR, kanR replicon: pCG2, pMB1 |
| Applications： | shuttle vector |
| Comments： | This vector contains the following restriction sites (in approximate kb from one PstI site): KpnI--0.8, 3.8; BamHI--4.4, 10.7, 13.4; HpaI--5.8; EcoRI--8.3; SalI--10.3; XhoI--12.5. The original vials from the depositor are being used for distribution. The pGA22 portion of the construct contains the drug resistance genes. cmlR can be insertionally inactivated by cloning into the EcoRI site; kanR can be inactivated by cloning into the HindIII or XhoI sites. Growth at 30C in the presence of chloramphenicol, kanamycin, or tetracycline was verified. Recommended tetracycline concentration is 1.5 ug/ml on LB agar. This was constructed by ligating pCG2 (6.6 kb) linearized with PstI to pGA22 (7.9 kb) linearized with PstI. |
| Media： | LB plus tetracycline (1.5 mcg/ml)
|
| Growth Conditions： | Temperature: 30.0°C |
| References： | Katsumata R, et al. Novel vector plasmids. US Patent 4,710,471 dated Dec 1 1987 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |