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| 产品名称： | YCp7-32cat |
|---|---|
| 商品货号： | TS182557 |
| Designations： | YCp7-32cat |
| Depositors： | H Feldmann |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.7600002288818360 Vector: YCp7-32cat (plasmid) Promoters: Promoter none Construction: YRp7-32cat (ATCC 37624), CEN6 Marker(s):TRP1,ampR Construct size (kb): 7.760000228881836 Features: insert detection: CAT marker(s): ampR, TRP1 promoter: none replicon: pMB1, ARS1 centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector promoter-cloning vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.2, 1.4, 0.56; PstI--6.5, 1.3; PvuII--6.0, 1.8. The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al. One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast. A 1520 bp NdeI fragment containing CEN6 was cloned into the NdeI site of YRp7-32cat (ATCC 37624). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257 |
| Shipped： | freeze-dried |