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| 产品名称： | YIp352 |
|---|---|
| 商品货号： | TS182563 |
| Designations： | YIp352 |
| Depositors： | A Tzagoloff |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.3000001907348630 Vector: YIp352 (plasmid) Promoters: Promoter lac Construction: YEp352 Marker(s):URA3,ampR Construct size (kb): 4.300000190734863 Features: insert detection: lacZ marker(s): ampR, URA3 promoter: lac replicon: pMB1 terminator: none enhancer: none |
| Applications： | YI-type (integrating) shuttle vector integrating vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.3; XbaI--4.3; PstI--4.3; HindIII--4.3. This is an Escherichia coli/Saccharomyces integrative shuttle vector with multiple cloning sites. Constructed from YEp352 (ATCC 37673) by cleavage at the SspI and HpaI sites in the 2 micron sequence. Some 2 micron sequence remains, but replicative function has been lost. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Hill JE, et al. Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 2: 163-167, 1986. PubMed: 3333305 Alexander Tzagoloff, personal communication |
| Shipped： | freeze-dried |