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| 产品名称： | pEA2 |
|---|---|
| 商品货号： | TS182617 |
| Designations： | pEA2 |
| Depositors： | SL Forsburg |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.8499999046325680 Vector: pEA2 (plasmid) Construction: pUC18, pMN1 Marker(s):ampR,his7+ Construct size (kb): 5.849999904632568 Features: marker(s): ampR marker(s): his7+ replicon: pMB1 MCS: SphI...EcoRI |
| Applications： | contains easily purifiable cassette(s) for construction |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--4.5, 1.4; HindIII--3.8, 1.3, 0.80; PstI--5.9. pEA2 carries his7+ cloned into the pUC18 polylinker. To construct gene disruptions from this vector, fragments carrying his7+ can be isolated using a variety of restriction enzymes. Constructed by inserting the 3.15 kb BglII fragment from plasmid pMN1 into the BamHI site of pUC18. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Apolinario E, et al. Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies. Curr. Genet. 24: 491-495, 1993. PubMed: 8299169 |
| Related Products： | component of:ATCC 87686 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried E. coli containing the plasmid |