| 产品名称: | pSVLfur-mu |
|---|---|
| 商品货号: | TS182638 |
| Designations: | pSVLfur-mu |
| GenBank Number: | L26489 |
| Species: | Mus musculus, mouse |
| Depositors: | WJ Van De Ven |
| Applications: | in another host, produces protein furin |
| Vector: | Construct size (kb): 7.650000095367432 |
| Insert: | DNA: cDNA Insert lengths(kb): 2.75 Gene product: furin Fur Target Gene: furin |
| Insert Size (kb): | 2.75 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): BamHI--6.9, 0.8; EcoRI--2.7 (doublet), 2.2; KpnI--5.6, 1.45, 0.6; NcoI--3.6, 1.4, 0.9, 0.75, 0.45, 0.3, 0.25; PstI--5.2, 2.5. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--1.99; KpnI--0.15, 0.73; NcoI--0.36, 0.80, 1.66, 2.61; NotI--0.16; PstI--2.47. The EcoRI insert site in pSVL was introduced in the SmaI site. |
| Classification: | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References: | Van de Ven WJ, et al. Furin: the prototype mammalian subtilisin-like proprotein-processing enzyme. Endoproteolytic cleavage at paired basic residues of proproteins of the eukaryotic secretory pathway. Enzyme 45: 257-270, 1991. PubMed: 1843280 Creemers JW, et al. Life Sci. Adv. 11: 127-138, 1992. Wim J Van De Ven, personal communication |