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| 产品名称： | pET-HisK |
|---|---|
| 商品货号： | TS182655 |
| Designations： | pET-HisK |
| Depositors： | T Hai |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.6750001907348630 Vector: pET-HisK (plasmid) Promoters: Promoter T7 (phi10) Construction: pET-His Marker(s):ampR Construct size (kb): 4.675000190734863 Features: marker(s): ampR other: NdeI/HMK/HMK/XhoI/NcoI/BamHI promoter: T7 (phi10) replicon: pMB1 terminator: phi10 |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.6; BamHI--4.6; BglI--2.5, 2.1, 0.25. The plasmid contains the following restriction sites (kb from nt 1): BamHI--4.16; BglI--0.87, 3.19, 3.42; BglII--3.99; EcoRI--4.67; HindIII--4.64; NcoI--4.16; NdeI--4.09; PstI--0.75; PvuI--0.62; PvuII--2.29; SalI--3.71; XbaI--4.05; XhoI--4.15. Digestion with NcoI, BamHI or XhoI and subsequent filling of ends, allows cloning of blunt-ended fragments with a +0, +1 (C) or +2 (GA) nucleotide reading frame, respectively. Expression vector for production of His-tagged fusion proteins, which contain two tandem phosphorylation sites, useful for radiolabeling. Fusion proteins can be purified using a nickel-chelating column, even under denaturing conditions. Constructed from pET-His (ATCC 87036) by insertion of two tandem consensus phosphorylation sites for heart muscle kinase (HMK) between the His coding region and the XhoI site of the polylinker. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Chen BP, Hai T. Expression vectors for affinity purification and radiolabeling of proteins using Escherichia coli as host. Gene 139: 73-75, 1994. PubMed: 8112591 |
| Shipped： | freeze-dried |