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| 产品名称： | HIBAA04 |
|---|---|
| 商品货号： | TS182749 |
| Designations： | HIBAA04 |
| GenBank Number： | M77836 T07966 T07967 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: lafmid BA Intact vector size: 3.153 Type of vector: phagemid Vector end: HindIII Vector end: NotI Cloning sites: HindIII, BamHI, NotI, EcoRI Polylinker sites: HindIII, BamHI, NotI, EcoRI Other unique sites: ClaI, XmnI, ScaI, ApaLI Construction: pGEM Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, insert detection: lacZ, primer site: M13 universal, -> MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098 primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: PYCR1 Genomic copy number: unique Gene name: pyrroline-5-carboxylate reductase 1 Contains complete coding sequence?: Y Chromosome: 17; Localization: 17 Tissue: infant brain, 3 months old (from Bento Soares) Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 2.0 Cross references: DNA Seq. Acc.: M77836 Nucleotides 46-1842 of the insert correspond to nucleotides 1-1792 of M77836 with 99% identity. DNA Seq. Acc.: T07966; EST: EST05857; Primer: M13-21; THC id: THC170714 DNA Seq. Acc.: T07967; EST: EST05858; Primer: M13 Reverse; THC id: THC170714 Insert lengths(kb): 2.0 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: pyrroline-5-carboxylate reductase 1( 1-pyrroline-5-carboxylate reductase, P5C reductase) PYCR1 |
| Insert Size (kb)： | 2.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.4; HindIII/NotI--3.4, 2.0. Based on the alignment with GenBank M77836 (1782 nt), this appears to be a full-length cDNA. |
| References： | Adams MD, et al. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat. Genet. 4: 373-380, 1993. PubMed: 8401585 |