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| 产品名称： | pCYM00 |
|---|---|
| 商品货号： | TS182935 |
| Designations： | pCYM00 |
| Depositors： | JH Camonis |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.4079999923706060 Vector: pCYM00 (plasmid) Promoters: Promoter SV40 early Construction: pCYM0 (ATCC 37820), pMC1403 Marker(s):URA3,ampR Construct size (kb): 9.407999992370605 Features: insert detection: lacZ marker(s): ampR, URA3 promoter: SV40 early replicon: pMB1, 2 micron terminator: SV40 |
| Applications： | YE-type (episomal) shuttle vector reporter construct shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/SalI--6.0(doublet); EcoRI--12.0; SmaI--12.0; Pvu/BamHI--6.5, 2.5, 2.1, 0.54, 0.49. Expresses beta-galactosidase activity in E. coli, S. cerevisiae, and mouse cells, and thus can be used as a control to calibrate transformation efficiency. Shuttle reporter plasmid. Constructed by inserting a BamHI/SalI fragment from pMC1403 (lac operon beginning at codon 9 of lacZ) between the BamHI/BalI (SalI-linkered) sites of pCYM0. Our plasmid is 12 kb instead of 9.4, which matches the original depositors DNA. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Camonis JH, et al. Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells. Gene 86: 263-268, 1990. PubMed: 2108907 |
| Shipped： | freeze-dried |