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| 产品名称： | pSV3-dhfr plasmid in Escherichia coli |
|---|---|
| 商品货号： | TS182978 |
| Designations： | pSV3-dhfr plasmid in Escherichia coli |
| Depositors： | P Berg, Stanford University Medical Center |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 |
| Vector Information： | Construct size (kb): 8.4
Vector: pSV3-dhfr (plasmid)
Construction: pBR322, SV40, mouse
Features: marker(s): ampR, mtxR other: SV40 large T antigen promoter: SV40 replicon: pMB1 |
| Applications： | Expression vector Shuttle vector |
| Comments： | Confers selective advantage to mammalian cell lines grown in the presence of methotrexate. Selectable marker in dhfr- cell culture cells. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Subramani S, et al. Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors. Mol. Cell. Biol. 1: 854-864, 1981. PubMed: 9279398 |
| Shipped： | freeze-dried |
| Shipping Information： | Plasmid in E. coli |