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U2OS-39 phage lysate

货号 TS183051
中文名称 null
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产品名称: U2OS-39 phage lysate
商品货号: TS183051
Designations: U2OS-39 phage lysate
GenBank Number:

M22489

Species: Homo sapiens, human
Depositors: Genetics Institute, Inc., JM Wozney
Vector: Vector: λ gt10
Vector type: bacteriophage
Vector size (kb): 43.340
Vector ends: EcoRI
Insert: Insert size (kb): 1.5
Gene: bone morphogenetic protein 2, BMP2
DNA: cDNA
Source: Human osteosarcoma cell line U-2-OS
Genbank accession: M22489
Insert ends: EcoRI
Insert Size (kb): 1.5
Media: ATCC® Medium 1592: SM buffer
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Freeze-dried bacteria-free lysate
Comments:
Restriction digests of the clone give the following sizes: EcoRI--32.5, 10.5, 1.5; BamHI--22.0, 15.0, 8.0, 7.0, 1.4; PstI--22.5, 6.0, 3.1, 2.5, 2.1, 1.75, 1.25, 1.2, 1.1, 0.9, 0.7, 0.6; HindIII--24.8, 10.0, 8.3, 4.8; SmaI--23.0, 9.0, 5.6, 2.4, 1.1, 1.0.
Contains the complete coding sequence, with 5 and 3 untranslated regions.
A 0.35 kb PvuII/XbaI fragment from the insert detects a genomic PstI fragment of 8.0 kb.
References:

Wozney JM, et al. Novel regulators of bone formation: molecular clones and activities. Science 242: 1528-1534, 1988. PubMed: 3201241

Tabas JA, et al. Bone morphogenetic protein: chromosomal localization of human genes for BMP1, BMP2A, and BMP3. Genomics 9: 283-289, 1991. PubMed: 2004778

Wang EA, et al. DNA sequences encoding osteoinductive products. US Patent 5,013,649 dated May 7 1991

Disclosure: This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.