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| 产品名称： | p13-2-21 |
|---|---|
| 商品货号： | TS183104 |
| Designations： | p13-2-21 |
| Species： | Homo sapiens, human |
| Depositors： | M Litt |
| Vector： | Construct size (kb): 5.71999979019165 |
| Insert： | DNA: genomic Insert lengths(kb): 0.5899999737739563 Gene product: DNA Segment, single copy D19S11 Alleles: F1, F2, A1, A1, A2, A2, B1, B2, C1, C2, D1, D2, D3 |
| Insert Size (kb)： | 0.590 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--5.72; PstI--5.72; HindIII--5.68, 0.4; EcoRI--5.6, 0.65; AvaI--5.72. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The BamHI RFLP is also detected by p13-1-82 (ATCC 59004, 59005). The combined heterozygosity of the polymorphisms detected by p13-1-82 (ATCC 59004, 59005) and p13-2-21 (TS183104, 59015) is 72%. Seven of 24 possible haplotypes occur with frequencies of 3-38%. p13-1-82 (ATCC 59004, 59005), p13-2-31 (TS183104, 59015), and p13-1-25 (ATCC 59870, 59871) were subcloned from cosmid 1-13 (C1-13). |
| References： | Bufton L, et al. Four restriction fragment length polymorphisms revealed by probes from a single cosmid map to chromosome 19. Am. J. Hum. Genet. 38: 447-460, 1986. PubMed: 3010711 Michael Litt, personal communication |