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微信小章| 产品名称： | HFBCP87 |
|---|---|
| 商品货号： | TS183120 |
| Designations： | HFBCP87 |
| GenBank Number： | M85923 |
| Species： | Homo sapiens, human |
| Applications： | The predicted product size is (bp): 107. The observed product size(s) is(are) (bp): 108 (human); 108 (CHO cells). |
| Vector： | Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D1S349E Genomic copy number: unique Gene name: DNA Segment, single copy, expressed Contains complete coding sequence?: N Chromosome: 1; Localization: 1 p31-p32 Genome: Homo sapiens Gene symbol: PDE4B Gene name: phosphodiesterase, cyclic AMP-specific (rolipram sensitive) Contains complete coding sequence?: N Chromosome: 1; Localization: 1 p31 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 2.0 Cross references: DNA Seq. Acc.: M85923; EST: EST02447; Primer: M13 Forward; THC id: THC106075 DNA Seq. Acc.: M97515; EST: EST02447; Primer: M13 Forward Nucleotides 1-367 of the insert correspond to nucleotides 687-1053 of M97515 with 98% identity. DNA Seq. Acc.: M97515; EST: EST02926; Primer: M13 Reverse Nucleotides 281-1 of the insert correspond to nucleotides 2366-2646 of M97515 with 98% identity. DNA Seq. Acc.: T05038; EST: EST02926; Primer: M13 Reverse; THC id: THC106075 Insert lengths(kb): 2.0 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: phosphodiesterase, cyclic AMP-specific (rolipram sensitive) PDE4B |
| Insert Size (kb)： | 2.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.0; BamHI--5.0; HindIII--3.15, 1.55, 0.3; PstI--5.0. Corresponds to nt 687-2646 of GenBank M97515, encoding part of the coding sequence (nt 282-1976) and part of the 3 untranslated region. EST02447 can be detected in genomic DNA using the following oligonucleotides (5-3): TCCAGGAAAGAGACCTCCTA and GTTGTGATATGCCACGTCAG. The predicted product size is (bp): 107. The observed product size(s) is(are) (bp): 108 (human); 108 (CHO cells). |
| References： | Korenberg JR, et al. Toward a cDNA map of the human genome. Genomics 29: 364-370, 1995. PubMed: 8666383 Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749 Maglott DR, et al259 human brain expressed sequence tags (ESTs): chromosome localization, subregional assignment, and sequence analysisIn: Maglott DR, et alIdentification of transcribed sequencesNew YorkPlenum Presspp. 273-288, 1994 Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434 |