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购买须知
| 产品名称： | Human herpesvirus 1 |
|---|---|
| 商品货号： | TS183135 |
| Classification： | Herpesviridae, Simplexvirus |
| Deposited As： | Herpes simplex type 1 |
| Agent： | Human herpesvirus 1 |
| Strain： | KOS |
| Common Name： | Herpes simplex virus 1 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | VR-1493™ was derived from VR-1487™ following treatment with a mycoplasma removal agent to remove mycoplasma contamination. Antigenically cross-reactive with Human herpesvirus 2. |
| Effect on Host： | CPE, syncytial formation, cell rounding, clumping, and sloughing |
| Recommended Host： | Vero (ATCC® CCL-81™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 1-3 days |
| Name of Depositor： | PA Schaffer, University of Pennsylvania |
| Special Collection： | NCRR Contract |
| Source： | Lip lesion of human with cold sore |
| References： | Smith KO. Relationship between the envelope and the infectivity of herpes simplex virus. Proc. Soc. Exp. Biol. Med. 115: 814-816, 1964. PubMed: 14155835 |