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| 产品名称： | Human echovirus 4 |
|---|---|
| 商品货号： | TS183167 |
| Classification： | Picornaviridae, Enterovirus, Human enterovirus B |
| Agent： | Human echovirus 4 |
| Strain： | Pesascek |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | This item is a mycoplasma free preparation derived from ATCC VR-34 following treatment to remove mycoplasma. VR-34 will no longer be distributed. |
| Product Format： | frozen |
| Storage Conditions： | xa0Store at -70°C or colder |
| Comments： | This item is a mycoplasma free preparation derived from ATCC VR-34 following treatment to remove mycoplasma. VR-34 will no longer be distributed. |
| Effect on Host： | Cytopathic effects in tissue culture CPE in the form of highly refractile, rounded cells throughout monolayer or in loose clusters. |
| Growth Medium： | ATCC® Medium 30-2003: Eagles Minimum Essential Medium (EMEM) Fetal Bovine Serum |
| Recommended Host： | Production Host: LLC-Mk2 derivative cells (ATCC CCL-7.1). Recommended Host: xa0LLC-Mk2 derivative cells (ATCC® CCL-7.1x99). Alternate Hosts: MkK, Mk (testis) and HED cells |
| Growth Conditions： | Plant cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI of about 0.1. Adsorb 1 hour at 37.0°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. |
| Name of Depositor： | J.L. Melnick |
| Source： | Fecal specimen from case of aseptic meningitis, Connecticut, 1951. |
| References： | Melnick J. Application of tissue culture methods to epidemiological studies of poliomyelitis. Am. J. Public Health 44: 571-580, 1954. PubMed: 13148397 |