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微信小章| 产品名称： | M158 |
|---|---|
| 商品货号： | TS183175 |
| Organism： | Mus musculus, mouse |
| Tissue： | breast; mammary gland |
| Cell Type： | epithelial cell |
| Product Format： | frozen |
| Morphology： | epithelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | breast cancer |
| Applications： | The cell line M158 overexpresses c-Myc. It is can be used to study the molecular mechanism and function of c-Myc in mammary gland tumors. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | The M158 cell line was derived from the mammary tumor of a transgenic mouse carrying the MMTV-c-myc transgene. |
| Clinical Data： | female |
| Genes Expressed： | Myc/c-Myc, expressed |
| Tumorigenic： | yes |
| Comments： | The M158 cell line was derived from the mammary tumor of a transgenic mouse carrying the MMTV-c-myc transgene.xa0
c-Myc plays a critical role in activating the transcription of growth-related genes.xa0 Elevated expression of c-Myc has been found in almost one-third of breast and colon carcinomas.xa0xa0 The cell line M158 overexpresses c-Myc. It is can be used to study the molecular mechanism and function of c-Myc in mammary gland tumors. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium renewal: Every 2 to 3 days. |
| Cryopreservation： | Freeze medium: complete growth medium supplemented with an additional 10% bovine calf serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°Cxa0
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | P Leder |
| References： | Stewart TA, et al. Spontaneous mammary adenocarcinomas in transgenic mice that carry and express MTV/myc fusion genes. Cell 38: 627-637, 1984 PubMed: 6488314 Elson A, et al. Protein-tyrosine phosphatase epsilon. An isoform specifically expressed in mouse mammary tumors initiated by v-Ha-ras OR neu. J. Biol. Chem. 270(44): 26116-26122, 1995 PubMed: 7592814 Amundadottir LT, et al. Signal transduction pathways activated and required for mammary carcinogenesis in response to specific oncogenes. Oncogene 16(6): 737-746, 1998 PubMed:9488037 Fantin VR, et al. A novel mitochondriotoxic small molecule that selectively inhibits tumor cell growth. Cancer Cell 2(1): 29-42, 2002 PubMed: 12150823 |